Jak2 [p Tyr1007, p Tyr1008] Antibody

Name: Jak2 [p Tyr1007, p Tyr1008] Antibody
Brand: Novus Biologicals
SKU: NBP3-13138
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-13138

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1007/Tyr1008 of human Jak2. The exact sequence is proprietary.

Modification

p Tyr1007, p Tyr1008

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Jak2 [p Tyr1007, p Tyr1008] Antibody

Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody [NBP3-13138]

Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody [NBP3-13138] - Untreated (-) and treated (+) K562 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Jak2 (phospho Tyr1007/1008) antibody (NBP3-13138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody [NBP3-13138] -

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with JAK2 (phospho Tyr1007/1008) antibody (NBP3-13138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Jak2 [p Tyr1007, p Tyr1008] Antibody

Application

Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Jak2

Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).

Long Name

Janus Kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2

Gene Symbol

JAK2

Additional Jak2 Products

Product Documents for Jak2 [p Tyr1007, p Tyr1008] Antibody

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Product Specific Notices for Jak2 [p Tyr1007, p Tyr1008] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.