JAM-B/VE-JAM Antibody - BSA Free

Name: JAM-B/VE-JAM Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-32655
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-32655

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human JAM-B/VE-JAM. The exact sequence is proprietary.

Reactivity Notes

Rat (81%).

Localization

Cell junction, tight junction, Cell membrane, Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JAM-B/VE-JAM Antibody - BSA Free

Western Blot: JAM-B/VE-JAM Antibody [NBP1-32655]

Western Blot: JAM-B/VE-JAM Antibody [NBP1-32655] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with JAM-B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655]

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655] - Paraffin-embedded TOV-112D xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655]

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655] - Paraffin-embedded human colon, using antibody at 1:200 dilution.

Applications for JAM-B/VE-JAM Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: JAM-B/VE-JAM

Junctional adhesion molecule 2 (JAM2), also know as JAMB, is a member of the newly reported glycoprotein family of adhesion molecules found at intercellular junctions of endothelial cells and lymphocytes (human). In adult murine tissue JAM2 expression is restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. Clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Leger, C.A. et al., 2002).

Long Name

Junctional Adhesion Molecule B

Alternate Names

CD322, JAM2, JAMB, VE-JAM

Gene Symbol

JAM2

Additional JAM-B/VE-JAM Products

Product Documents for JAM-B/VE-JAM Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for JAM-B/VE-JAM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.