Japanese Encephalitis virus NS5 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-13016
![Western Blot: Japanese Encephalitis virus NS5 Antibody [NBP3-13016]](https://resources.rndsystems.com/images/products/Japanese-Encephalitis-virus-NS5-Antibody-Western-Blot-NBP3-13016-img0001.jpg "Western Blot: Japanese Encephalitis virus NS5 Antibody [NBP3-13016]")
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of NS5 (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.
Reactivity Notes
Japanese encephalitis virus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Japanese Encephalitis virus NS5 Antibody - BSA Free
Western Blot: Japanese Encephalitis virus NS5 Antibody [NBP3-13016]
Western Blot: Japanese Encephalitis virus NS5 Antibody [NBP3-13016] - NS5 (JEV) antibody detects NS5 (JEV) protein by Western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NS5 (JEV) antibody (NBP3-13016) at a dilution of 1:3000 and developed with Trident femto Western HRP Substrate.![Immunocytochemistry/ Immunofluorescence: Japanese Encephalitis virus NS5 Antibody [NBP3-13016]](https://resources.rndsystems.com/images/products/Japanese-Encephalitis-virus-NS5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13016-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Japanese Encephalitis virus NS5 Antibody [NBP3-13016]")
Immunocytochemistry/ Immunofluorescence: Japanese Encephalitis virus NS5 Antibody [NBP3-13016]
Immunocytochemistry/Immunofluorescence: Japanese Encephalitis virus NS5 Antibody [NBP3-13016] - NS5 protein (Japanese encephalitis virus) antibody detects NS5 protein (Japanese encephalitis virus) by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol. Green: NS5 (Japanese encephalitis virus) protein stained by NS5 protein (Japanese encephalitis virus) antibody (NBP3-13016) diluted at 1:3000. Blue: Hoechst 33342 staining.Applications for Japanese Encephalitis virus NS5 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Japanese Encephalitis virus NS5
Alternate Names
JEV NS5, Non-structural protein 5, RNA-directed RNA polymerase NS5
Additional Japanese Encephalitis virus NS5 Products
Product Documents for Japanese Encephalitis virus NS5 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Japanese Encephalitis virus NS5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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