JMJD1B Antibody (1A8)

Western Blot: JMJD1B Antibody (1A8) [NBP3-32491]

Western Blot: JMJD1B Antibody (1A8) [NBP3-32491] - Western blot analysis of JMJD1B on different lysates with Rabbit anti-JMJD1B antibody (NBP3-32491) at 1/1,000 dilution.

Lane 1: Hela cell lysate (25 ug/Lane)
Lane 2: NIH/3T3 cell lysate (10 ug/Lane)

Predicted band size: 191 kDa
Observed band size: 191 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32491) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: JMJD1B Antibody (1A8) [NBP3-32491]

Immunohistochemistry: JMJD1B Antibody (1A8) [NBP3-32491] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-JMJD1B antibody (NBP3-32491) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32491) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: JMJD1B Antibody (1A8) [NBP3-32491]

Immunocytochemistry/ Immunofluorescence: JMJD1B Antibody (1A8) [NBP3-32491] - Immunocytochemistry analysis of Hela cells labeling JMJD1B with Rabbit anti-JMJD1B antibody (NBP3-32491) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-JMJD1B antibody (NBP3-32491) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.