JMJD2B Antibody (8I0I1)

Novus Biologicals | Catalog # NBP3-16639

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8I0I1 expressed in HEK293
View all JMJD2B Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human JMJD2B (O94953). PPKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JMJD2B Antibody (8I0I1)

Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639]

Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639]

Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639] - Western blot analysis of extracts of various cell lines, using JMJD2B Rabbit mAb (NBP3-16639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunocytochemistry/Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of U-2 OS cells using JMJD2B Rabbit mAb (NBP3-16639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunocytochemistry/Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of C6 cells using JMJD2B Rabbit mAb (NBP3-16639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunoprecipitation analysis of 300ug extracts of HCT116 cells using 3ug JMJD2B antibody (NBP3-16639). Western blot was performed from the immunoprecipitate using JMJD2B antibody (NBP3-16639) at a dilition of 1:1000.
JMJD2B Antibody (8I0I1)

Chromatin Immunoprecipitation- JMJD2B Antibody (8I0I1) [NBP3-16639] -

Chromatin Immunoprecipitation- JMJD2B Antibody (8I0I1) [NBP3-16639] - Analysis of extracts of 5637; cells, using KDM4B/JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
JMJD2B Antibody (8I0I1)

Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] -

Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of U-2 OS cells using JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
JMJD2B Antibody (8I0I1)

Chromatin Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639] -

Chromatin Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639] - Chromatin immunoprecipitation analysis of extracts of 5637; cells, using JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
JMJD2B Antibody (8I0I1)

Chromatin Immunoprecipitation: JMJD2B Antibody (8I0I1) [JMJD2B] -

Chromatin Immunoprecipitation: JMJD2B Antibody (8I0I1) [JMJD2B] - Chromatin immunoprecipitation analysis of extracts of 5637; cells, using JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for JMJD2B Antibody (8I0I1)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JMJD2B

JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.

Long Name

Lysine-specific demethylase 4B

Alternate Names

EC 1.14.11.66, JHDM3B, KDM4B, KIAA0876

Gene Symbol

KDM4B

Additional JMJD2B Products

Product Documents for JMJD2B Antibody (8I0I1)

Certificate of Analysis

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Product Specific Notices for JMJD2B Antibody (8I0I1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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