KAT2A/GCN5 Antibody

Novus Biologicals | Catalog # NBP2-17017

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (96%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human KAT2A/GCN5. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit KAT2A/GCN5 Antibody (NBP2-17017) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for KAT2A/GCN5 Antibody

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP2-17017]

Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP2-17017]

Immunocytochemistry/Immunofluorescence: KAT2A/GCN5 Antibody [NBP2-17017] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.
Western Blot: KAT2A/GCN5 Antibody [NBP2-17017]

Western Blot: KAT2A/GCN5 Antibody [NBP2-17017]

Western Blot: KAT2A/GCN5 Antibody [NBP2-17017] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: KAT2A/GCN5 Antibody [NBP2-17017]

Western Blot: KAT2A/GCN5 Antibody [NBP2-17017]

Western Blot: KAT2A/GCN5 Antibody [NBP2-17017] - 293T whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCN5 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
KAT2A/GCN5 Antibody

Western Blot: KAT2A/GCN5 Antibody [NBP2-17017] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCN5 antibody [C2C3], C-term (NBP2-17017) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KAT2A/GCN5 Antibody

Immunohistochemistry-Paraffin: KAT2A/GCN5 Antibody [NBP2-17017] -

GCN5 antibody [C2C3], C-term detects GCN5 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
GCN5 stained by GCN5 antibody [C2C3], C-term (NBP2-17017) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for KAT2A/GCN5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KAT2A/GCN5

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM]

Long Name

Lysine Acetyltransferase 2A

Alternate Names

GCN5L2, HGCN5, KAT2A, PCAF-B

Gene Symbol

KAT2A

UniProt

Additional KAT2A/GCN5 Products

Product Documents for KAT2A/GCN5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for KAT2A/GCN5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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