Ki67/MKI67 Antibody - BSA Free

Novus Biologicals | Catalog # NB110-90592

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human, Mouse, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ki67/MKI67 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Human KI67/MKI67, between amino acids 1200-1300 [Uniprot: P46013].

Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:32647136).

Localization

Nuclear

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

359 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ki67/MKI67 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB110-90592 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB110-89717 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Ki67-MKI67-Antibody-Immunohistochemistry-NB110-90592-img0006.jpg
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Ki-67/MKI67 (NB110-90592) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Detection of human lymph node. (20X)
Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-90592]

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Detection of human lymph node. (40X)
Ki67/MKI67 Antibody - BSA Free

Ki67/MKI67 in A431 Human Cell Line.

Ki67/MKI67 was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-Ki67/MKI67 Affinity Purified Polyclonal Antibody conjugated to Biotin (Catalog # NB110-90592B) at 2 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for Ki67/MKI67 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:3200

Immunohistochemistry-Frozen

reported in scientific literature (PMID 25647012)

Immunohistochemistry-Paraffin

1:3200. Use reported in scientific literature (PMID 24599134)

Reviewed Applications

Read 1 review rated 4 using NB110-90592 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Entrez Gene IDs

4288 (Human)

Gene Symbol

MKI67

UniProt

P46013 (Human)

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ki67/MKI67 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

Citations for Ki67/MKI67 Antibody - BSA Free

Customer Reviews for Ki67/MKI67 Antibody - BSA Free (1)

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  • Name: Kelsey Stockbridge
    Application: Immunofluorescence
    Sample Tested: mouse small intestinal epithelial cell line
    Species: Mouse
    Verified Customer | Posted 12/22/2010

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Protocols

View specific protocols for Ki67/MKI67 Antibody - BSA Free (NB110-90592):


Immunohistochemistry

1. Slice fresh tissues in 3um sections
2. Formaldehyde fixation 50C for 1 hour
3. Dehydrate under 80%, 90%, 90%, 95%, 95%, 100% and 100% ethanol successively under 50C for 1 hour of each concentration
4. Xylene I 40C for 1 hour
5. Xylene II 40C for 1 hour
6. Paraffin I embedding 65 C for 1 hour
7. Paraffin II embedding 65 C for 1 hour
8. Paraffin III embedding 65 C for 1 hour
9. Deparaffinize and rehydrate sections as standard
10. Antigen retrieval using 10mM sodium citrate microwave for 20 minutes
11. Wash and PBS wash as standard
12. Add 100ul primary antibody diluted to 1:3200 separately and incubate at 4C overnight
13. Wash in PBS buffer for 3 times 5 minutes
14. Remove excess PBS buffer and incubate sections with biotinylated secondary antibody, incubate for 30 minutes at 30C
15. Wash in PBS buffer for 3 times 5 minutes
16. Remove excess PBS buffer and incubate sections with ABC at 30C for 30 minutes
17. Wash in PBS buffer for 3 times 5 minutes
18. Develop with DAB
19. Rinse slides in gently running tap water for 10 minutes

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FAQs for Ki67/MKI67 Antibody - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Is there a clone number for this Ki67 antibody?

    A: This antibody is polyclonal, and thus it does not have a clone number.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A: The concentration is lot specific available upon request.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A:  Optimal concentrations and conditions for each application should be determined by the user.

  • Q: Is there a clone number for this Ki67 antibody?

    A: This antibody is polyclonal, and thus it does not have a clone number.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A: The concentration is lot specific available upon request.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A:  Optimal concentrations and conditions for each application should be determined by the user.

  • Q: Is there a clone number for this Ki67 antibody?

    A: This antibody is polyclonal, and thus it does not have a clone number.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A: The concentration is lot specific available upon request.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A:  Optimal concentrations and conditions for each application should be determined by the user.

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