Kinesin 5B Antibody (JE64-16)

Western Blot: Kinesin 5B Antibody (JE64-16) [NBP3-32503]

Western Blot: Kinesin 5B Antibody (JE64-16) [NBP3-32503] - Western blot analysis of Kinesin 5B on different lysates with Rabbit anti-Kinesin 5B antibody at 1/500 dilution.

Lane 1: HeLa cell lysate, 10 ug/Lane
Lane 2: A549 cell lysate, 10 ug/Lane
Lane 3: Mouse kidney tissue lysate, 20 ug/Lane

Predicted band size: 110 kDa
Observed band size: 110 kDa

Exposure time: 1 minute;

8 % SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody KIF5B at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Kinesin 5B Antibody (JE64-16) [NBP3-32503]

Immunohistochemistry: Kinesin 5B Antibody (JE64-16) [NBP3-32503] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-Kinesin 5B antibody (NBP3-32503) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody KIF5B at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.