Kinesin 5B Antibody - BSA Free

Novus Biologicals | Catalog # NB500-580

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Porcine

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Kinesin 5B Primary Antibodies »

Product Specifications

Immunogen

Stalk domain of human kinesin (amino acids 331-906) expressed in E. coli (FKHC3).

Specificity

This detects total level of endogenous kinesin protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kinesin 5B Antibody - BSA Free

Western Blot: Kinesin 5B AntibodyBSA Free [NB500-580]

Western Blot: Kinesin 5B AntibodyBSA Free [NB500-580]

Western Blot: Kinesin Antibody [NB500-580] - Detection of kinesin in reduced lysates of human HEK cell line (1) and porcine brain (2) by rabbit polyclonal anti-kinesin.

Applications for Kinesin 5B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:1000
Application Notes
Predicted molecular weight: 110 kDa. Purity is > 95% (by SDS-PAGE)

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Kinesin 5B

Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N terminal globular motordomain with both a microtubule binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Alternate Names

Conventional kinesin heavy chain, kinesin family member 5B, kinesin-1 heavy chain, KNS1kinesin 1 (110-120kD), KNSKINH, Ubiquitous kinesin heavy chain, UKHCkinesin heavy chain

Entrez Gene IDs

3799 (Human); 16573 (Mouse)

Gene Symbol

KIF5B

Additional Kinesin 5B Products

Product Documents for Kinesin 5B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Kinesin 5B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Kinesin 5B Antibody - BSA Free

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Protocols

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