KISS1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-34010

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Metastin/KiSS1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: CTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KISS1 Antibody - BSA Free

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010] - Analysis in human placenta and skeletal muscle tissues. Corresponding KISS1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: KISS1 Antibody [NBP2-34010]

Immunocytochemistry/ Immunofluorescence: KISS1 Antibody [NBP2-34010]

Immunocytochemistry/Immunofluorescence: KISS1 Antibody [NBP2-34010] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010] - Staining of human testis shows moderate perinuclear positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010]

Immunohistochemistry-Paraffin: KISS1 Antibody [NBP2-34010] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for KISS1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Metastin/KiSS1

KISS1 gene encodes metastin, also known as kisspeptin or KiSS-1 peptide, which binds to a G protein-coupled receptor GPR54. KISS1 is dominantly expressed in brain (e.g. anteroventral periventricular nucleus, AVPV arcuate nucleus, ARC), placenta, and pancreas, etc. Metastin plays an important role in regulating the GnRH secretion in brain and MMP-9 expression in placenta, which is associated with development of puberty and sex maturation (e.g. Idiopathic hypogonadotropic hypogonadism, IHH) as well as placentation (e.g. pre-eclampsia), respectively. Metastin was originally discovered as a tumor metastasis suppressor (e.g. melanoma, breast cancer).

Long Name

KiSS-1 Metastasis-suppressor

Alternate Names

KiSS1, Kisspeptin-1

Gene Symbol

KISS1

UniProt

Q15726 (Human)

Additional Metastin/KiSS1 Products

Product Documents for KISS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KISS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for KISS1 Antibody - BSA Free

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Protocols

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FAQs

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