KLF5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32245

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (96%), Porcine (96%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KLF5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLF5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: KLF5 Antibody [NBP1-32245]

Immunocytochemistry/ Immunofluorescence: KLF5 Antibody [NBP1-32245]

Immunocytochemistry/Immunofluorescence: KLF5 Antibody [NBP1-32245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: KLF5 stained by KLF5 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245] - Mouse testis. KLF5 stained by KLF5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: KLF5 Antibody [NBP1-32245]

Western Blot: KLF5 Antibody [NBP1-32245]

Western Blot: KLF5 Antibody [NBP1-32245] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: KLF5 Antibody [NBP1-32245]

Western Blot: KLF5 Antibody [NBP1-32245]

Western Blot: KLF5 Antibody [NBP1-32245] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 Antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: KLF5 Antibody [NBP1-32245]

Western Blot: KLF5 Antibody [NBP1-32245]

Western Blot: KLF5 Antibody [NBP1-32245] - Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245] - HeLa xenograft. KLF5 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245] - Mouse intestine. KLF5 stained by KLF5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
KLF5 Antibody

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245] -

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-32245] - Mouse colon.KLF5 stained by KLF5 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
KLF5 Antibody

Western Blot: KLF5 Antibody [NBP1-32245] -

Western Blot: KLF5 Antibody [NBP1-32245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (NBP1-32245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KLF5 Antibody - BSA Free

Western Blot: KLF5 Antibody [NBP1-32245] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (NBP1-32245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KLF5 Antibody - BSA Free

Western Blot: KLF5 Antibody [NBP1-32245] -

HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (NBP1-32245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for KLF5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KLF5

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor.

Long Name

Kruppel-Like Factor 5

Alternate Names

BTEB2

Gene Symbol

KLF5

Additional KLF5 Products

Product Documents for KLF5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for KLF5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KLF5 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for KLF5 Antibody - BSA Free

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  • Q: Our customer would like to know the concentration of NBP1-32245 from the latest lot available.

    A: The KLF5 antibody with catalogue number NBP1-32245 is supplied at a concentration of 1mg/ml.

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