KLF8 Antibody

Novus Biologicals | Catalog # NB100-1050

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all KLF8 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence VDMDKLINNLEVQ-C corresponding to N-Terminus according to NP_009181.2, NP_001152768.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Porcine.

Specificity

This antibody is expected to recognise isoform 1 (NP_009181.2) and isoform 2 (NP_001152768.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for KLF8 Antibody

Western Blot: KLF8 Antibody [NB100-1050]

Western Blot: KLF8 Antibody [NB100-1050]

Western Blot: KLF8 Antibody [NB100-1050] - (2ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence..

Applications for KLF8 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human kidney lysates is reported in scientific literature (PMID: 15557280).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLF8

Kruppel-like factor proteins all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers.

Alternate Names

Basic krueppel-like factor 3, basic kruppel-like factor 3, BKLF3DKFZp686O08126, DXS741, Krueppel-like factor 8, Kruppel-like factor 8, Zinc finger protein 741, ZNF741MGC138314

Entrez Gene IDs

11279 (Human)

Gene Symbol

KLF8

UniProt

NP_009181 (Human)

Additional KLF8 Products

Product Documents for KLF8 Antibody

Certificate of Analysis

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Product Specific Notices for KLF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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