KLF9 Antibody (OTI8A11) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70285

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8A11

Format

Azide and BSA Free
View all KLF9 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human KLF9(NP_001197) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLF9 Antibody (OTI8A11) - Azide and BSA Free

Western Blot: KLF9 Antibody (OTI8A11)Azide and BSA Free [NBP2-70285]

Western Blot: KLF9 Antibody (OTI8A11)Azide and BSA Free [NBP2-70285]

Western Blot: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF9.
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Adenocarcinoma of Human endometrium tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285]

Immunohistochemistry: KLF9 Antibody (OTI8A11) - Azide and BSA Free [NBP2-70285] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for KLF9 Antibody (OTI8A11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KLF9

KLF9 is a gene that codes for a transcription factor that activates mRNA synthesis on a selective basis from genes that only contain tandem repeats of GC boxes, otherwise the gene will be suppressed, and it has a length of 244 amino acids, and amass of approximately 27 kDa. Studies have been conducted on diseases and disorders relating to this gene, including Kabuki syndrome, otosclerosis, leiomyoma, colorectal cancer, carcinoma, glioblastoma, cholesterol, thyroiditis, and leukemia. KLF9 has also been shown to have interactions with PGR, SP1, SIN3A, HDAC7, and ELAV1 in pathways such as the diurnally regulated gene pathway.

Alternate Names

basic transcription element binding protein 1, Basic transcription element-binding protein 1, BTEB1GC-box-binding protein 1, BTEBBTE-binding protein 1, Krueppel-like factor 9, Kruppel-like factor 9, Transcription factor BTEB1

Gene Symbol

KLF9

Additional KLF9 Products

Product Documents for KLF9 Antibody (OTI8A11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for KLF9 Antibody (OTI8A11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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