KLHL2 Antibody (OTI1G7)

Novus Biologicals | Catalog # NBP2-02939

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G7
View all KLHL2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

65.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLHL2 Antibody (OTI1G7)

Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] Analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Immunocytochemistry/Immunofluorescence: KLHL2 Antibody (1G7) [NBP2-02939] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2.
Flow Cytometry: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Flow Cytometry: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Flow Cytometry: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody, and then analyzed by flow cytometry.
Western Blot: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Western Blot: KLHL2 Antibody (OTI1G7) [NBP2-02939]

Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.

Applications for KLHL2 Antibody (OTI1G7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLHL2

KLHL2, also known as Kelch-like protein 2, has a 593 short amino acid that is 66 kDa and a long 597 amino acid that is 67 kDa, predominantly expressed in brain, and may participate in organizing the actin cytoskeleton of the brain cells. This protein is currently being studied for research on the following diseases and disorders: contagious pustular dermatitis, lumpy skin disease, monkeypox, smallpox, cowpox, vaccinia, skin disease, dermatitis, pharyngitis, breast cancer, and neuronitis. The protein has been shown to interact with KLHL12 in intracellular protein transport pathways.

Alternate Names

ABP-KELCH, Actin-binding protein Mayven, kelch, kelch (Drosophila)-like 2 (Mayven), kelch-like 2, Mayven (Drosophila), kelch-like protein 2, MAV, MAYVEN

Entrez Gene IDs

11275 (Human)

Gene Symbol

KLHL2

Additional KLHL2 Products

Product Documents for KLHL2 Antibody (OTI1G7)

Certificate of Analysis

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Product Specific Notices for KLHL2 Antibody (OTI1G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KLHL2 Antibody (OTI1G7)

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Protocols

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