KLHL25 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77147
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the center of human ENC-2. The immunogen is located within amino acids 230 - 280 of ENC-2. Amino Acid Squence: SSEALMADERTKL
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Scientific Data Images for KLHL25 Antibody - BSA Free
Western Blot: KLHL25 AntibodyBSA Free [NBP1-77147]
Western Blot: KLHL25 Antibody [NBP1-77147] - Jurkat cell lysate with KLHL25 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody - BSA Free [NBP1-77147] -
Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody - BSA Free [NBP1-77147] - Immunocytochemistry of KLHL25 in Jurkat cells with KLHL25 antibody at 5 u/mL.Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody - BSA Free [NBP1-77147] -
Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody - BSA Free [NBP1-77147] - Immunofluorescence of KLHL25 in Jurkat cells with KLHL25 antibody at 20 u/mL.Applications for KLHL25 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
5-20 ug/ml
Western Blot
1 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KLHL25
Alternate Names
ectodermal-neural cortex 2, ENC-2BTB/POZ KELCH domain protein, ENC2ectoderm-neural cortex protein 2, FLJ12587, FLJ45015, kelch-like 25 (Drosophila), Kelch-like protein 25
Gene Symbol
KLHL25
UniProt
Additional KLHL25 Products
Product Documents for KLHL25 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for KLHL25 Antibody - BSA Free
Chicken products cannot be exported to Canada.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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