KMT2B Antibody

Western Blot: KMT2B Antibody [NBP3-18667]

Western Blot: KMT2B Antibody [NBP3-18667] - Figure 2. Western blot analysis using the antibody directed against KMT2B Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the antibody against mouse KMT2B, diluted 1:500 in BSA/PBS-Tween. The location of the N-terminal fragment of KMT2B is indicated on the right (270 kDa). Cells homozygous for the targeted conditional mll2 allele (delta KMT2B) show a dramatic reduction of KMT2B protein after partial recombination, whereas KMT2B protein was detected in deltaMll1, deltaMll4 and WT E14Tg2a cells.

ELISA: KMT2B Antibody [NBP3-18667]

ELISA: KMT2B Antibody [NBP3-18667] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse KMT2B. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:32,500.