KPNA5 Antibody (1D2) - Azide and BSA Free

Novus Biologicals | Catalog # H00003841-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D2

Format

Azide and BSA Free
View all KPNA5 Primary Antibodies »

Product Specifications

Immunogen

KPNA5 (AAH47409.1, 1 a.a. ~ 539 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKRRNVYLPRNDESMLESPIQDPDISSTVPIPEEEVVTTDMVQMIFSNNADQQLTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCTLQFEAAWALTNIASGTFLHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEILPPLLELLTNSNRLTTTRNAVWALSNLCRGKNPPPNFSKVSPCLNVLSRLLFSSDPDVLADVCWALSYLSDGPNDKIQAVIDSGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSSPKESIRKEACWTVSNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYLVALGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQESKQNGIGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGVEEDDPSIVPQVDENQQQFIFQQQEAPMDGFQL

Localization

Cytoplasmic

Specificity

KPNA5 - karyopherin alpha 5 (importin alpha 6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for KPNA5 Antibody (1D2) - Azide and BSA Free

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01]

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01]

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - Analysis of KPNA5 expression in transfected 293T cell line by KPNA5 monoclonal antibody (M01), clone 1D2.Lane 1: KPNA5 transfected lysate(60.7 KDa).Lane 2: Non-transfected lysate.
Western Blot: KPNA5 Antibody (1D2) [H00003841-M01]

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01]

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - Analysis of KPNA5 over-expressed 293 cell line, cotransfected with KPNA5 Validated Chimera RNAi ( Cat # H00003841-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KPNA5 monoclonal antibody (M01), clone 1D2 (Cat # H00003841-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: KPNA5 Antibody (1D2) [H00003841-M01]

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01]

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - KPNA5 monoclonal antibody (M01), clone 1D2 Analysis of KPNA5 expression in HepG2.
ELISA: KPNA5 Antibody (1D2) [H00003841-M01]

ELISA: KPNA5 Antibody (1D2) [H00003841-M01]

ELISA: KPNA5 Antibody (1D2) [H00003841-M01] - Detection limit for recombinant GST tagged KPNA5 is approximately 3ng/ml as a capture antibody.

Applications for KPNA5 Antibody (1D2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA. Use in immunoprecipitation reported in scientific literature (PMID: 26052702).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KPNA5

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus.

Alternate Names

importin alpha 6, importin subunit alpha-6, IPOA6, karyopherin alpha 5 (importin alpha 6), Karyopherin subunit alpha-5, SRP6

Entrez Gene IDs

3841 (Human)

Gene Symbol

KPNA5

OMIM

604545 (Human)

UniProt

AAH47409 (Human)

Additional KPNA5 Products

Product Documents for KPNA5 Antibody (1D2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KPNA5 Antibody (1D2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KPNA5 Antibody (1D2) - Azide and BSA Free

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