KRT9 Antibody (SD201-09)
Novus Biologicals | Catalog # NBP2-67810
Recombinant Monoclonal Antibody
![Western Blot: KRT9 Antibody (SD201-09) [NBP2-67810]](https://resources.rndsystems.com/images/products/KRT9-Antibody-SD201-09-Western-Blot-NBP2-67810-img0002.jpg "Western Blot: KRT9 Antibody (SD201-09) [NBP2-67810]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SD201-09 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein wiehin Human KRT9 aa 234-422 / 623. (SwissProt: P35527 Human; SwissProt: Q6RHW0 Mouse; SwissProt: Q8CIS9 Rat)
Localization
Extracellular exosome, membrane, nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KRT9 Antibody (SD201-09)
Western Blot: KRT9 Antibody (SD201-09) [NBP2-67810]
Western Blot: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of KRT9 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.![Immunocytochemistry/ Immunofluorescence: KRT9 Antibody (SD201-09) [NBP2-67810]](https://resources.rndsystems.com/images/products/KRT9-Antibody-SD201-09-Immunocytochemistry-Immunofluorescence-NBP2-67810-img0003.jpg "Immunocytochemistry/ Immunofluorescence: KRT9 Antibody (SD201-09) [NBP2-67810]")
Immunocytochemistry/ Immunofluorescence: KRT9 Antibody (SD201-09) [NBP2-67810]
Immunocytochemistry/Immunofluorescence: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of MCF-7 cells labeling KRT9 (and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody KRT9 (red) at 1/200 dilution for overnight at 4. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibody at 1/800 dilution. DAPI was used to stain the cell nuclei (blue).![Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]](https://resources.rndsystems.com/images/products/KRT9-Antibody-SD201-09-Immunohistochemistry-Paraffin-NBP2-67810-img0005.jpg "Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]")
Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]
Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling KRT9 (washed with PBS. And then probed with the primary antibodies KRT9 (red) at 1/50 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).![Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]](https://resources.rndsystems.com/images/products/KRT9-Antibody-SD201-09-Immunohistochemistry-Paraffin-NBP2-67810-img0001.jpg "Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]")
Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]
Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of mouse skin tissue using anti-Cytokeratin 9 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]](https://resources.rndsystems.com/images/products/KRT9-Antibody-SD201-09-Immunohistochemistry-Paraffin-NBP2-67810-img0004.jpg "Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]")
Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810]
Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling KRT9 washed with PBS. And then probed with the primary antibodies KRT9 (red) at 1/50 dilution and Vimentin (green) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).Applications for KRT9 Antibody (SD201-09)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KRT9
Alternate Names
cytokeratin-9, EPPK, keratin 9, keratin, type I cytoskeletal 9
Gene Symbol
KRT9
Additional KRT9 Products
Product Documents for KRT9 Antibody (SD201-09)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for KRT9 Antibody (SD201-09)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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