Key Product Details

Species Reactivity

Human, Chicken (Negative), Mouse (Negative), Primate, Rat (Negative)

Applications

Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KU729

Format

Azide and BSA Free
View all Ku70/XRCC6 Primary Antibodies »

Product Specifications

Immunogen

Nuclear extract of human HL-60 cells

Reactivity Notes

Does not react with Mouse, Rat and Chicken.

Localization

Nuclear

Marker

Nuclear Marker

Specificity

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This monoclonal antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34247).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.
Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Alexa Fluor 700 format of this antibody.
Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku70/XRCC6 Antibody (KU729).
Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663]

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku70/XRCC6 Antibody (KU729).

Applications for Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1.0ug/ml
Application Notes
Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Ku70/XRCC6

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Long Name

Lupus Ku Autoantigen Protein 70

Alternate Names

CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6

Gene Symbol

XRCC6

Additional Ku70/XRCC6 Products

Product Documents for Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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