Ku70/XRCC6 Antibody (KU729)

Novus Biologicals | Catalog # NBP2-34247

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Chicken (Negative), Mouse (Negative), Primate, Rat (Negative)

Applications

Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KU729
View all Ku70/XRCC6 Primary Antibodies »

Product Specifications

Immunogen

Nuclear extract of human HL-60 cells

Reactivity Notes

Does not react with Mouse, Rat and Chicken.

Localization

Nuclear

Marker

Nuclear Marker

Specificity

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This monoclonal antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34663)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Ku70/XRCC6 Antibody (KU729)

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247]

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247]

Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Immunofluorescent analysis of HeLa cells using Ku70/XRCC6 antibody (KU729). Image from verified customer review.
Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247]

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247]

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku70/XRCC6 Antibody (KU729).
Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247]

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247]

Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.
Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247]

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247]

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku70/XRCC6 Antibody (KU729).
Ku70/XRCC6 Antibody (KU729)

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] -

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Ku70/XRCC6 Antibody (KU729)

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] -

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for Ku70/XRCC6 Antibody (KU729)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Immunocytochemistry (Acetone-fixed cells): 0.5-1.0ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34247 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Ku70/XRCC6

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Long Name

Lupus Ku Autoantigen Protein 70

Alternate Names

CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6

Gene Symbol

XRCC6

UniProt

P12956, P13010

Additional Ku70/XRCC6 Products

Product Documents for Ku70/XRCC6 Antibody (KU729)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ku70/XRCC6 Antibody (KU729)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Ku70/XRCC6 Antibody (KU729) (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Ku70/XRCC6 Antibody (KU729)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Ku70/XRCC6 Antibody (KU729)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 03/22/2022
    HeLa cells
    Ku70/XRCC6 Antibody (KU729) NBP2-34247

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies