L3MBTL3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-47316
![Immunohistochemistry: L3MBTL3 Antibody [NBP1-47316]](https://resources.rndsystems.com/images/products/L3MBTL3-Antibody-Immunohistochemistry-NBP1-47316-img0002.jpg "Immunohistochemistry: L3MBTL3 Antibody [NBP1-47316]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 550 and 600 of human Lethal(3)Malignant Brain Tumor-like 3 using the numbering given in entry NP_115814.1 (GeneID 84456).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for L3MBTL3 Antibody - BSA Free
Immunohistochemistry: L3MBTL3 Antibody [NBP1-47316]
Immunohistochemistry: L3MBTL3 Antibody [NBP1-47316] - Detection of human L3MBTL3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- L3MBTL3 (NBP1-47316) used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate![Immunoprecipitation: L3MBTL3 Antibody [NBP1-47316]](https://resources.rndsystems.com/images/products/L3MBTL3-Antibody-Immunoprecipitation-NBP1-47316-img0001.jpg "Immunoprecipitation: L3MBTL3 Antibody [NBP1-47316]")
Immunoprecipitation: L3MBTL3 Antibody [NBP1-47316]
Immunoprecipitation: L3MBTL3 Antibody [NBP1-47316] - Detection of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-L3MBTL3 antibody NBP1-47316 used for IP at 3 ug/mg lysate. L3MBTL3 was also immunoprecipitated by rabbit anti-L3MBTL3 antibodies A302-852A and NBP1-47315, which recognize upstream epitopes. For blotting immunoprecipitated L3MBTL3, NBP1-47314 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.PreviousApplications for L3MBTL3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunoprecipitation
2-5 ug/mg lysate
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: L3MBTL3
Long Name
L3MBTL Histone Methyl-Lysine Binding Protein 3
Alternate Names
KIAA1798, MBT-1
Entrez Gene IDs
84456 (Human)
Gene Symbol
L3MBTL3
UniProt
Additional L3MBTL3 Products
Product Documents for L3MBTL3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for L3MBTL3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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