LacI Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77854

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Bacteria, E. coli

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LacI Primary Antibodies »

Product Specifications

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.

Reactivity Notes

Reactive in E.coli and S.sonnei

Specificity

This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LacI Antibody - BSA Free

LacI Antibody

Western Blot: LacI Antibody [NBP1-77854] -

Lane 1: Molecular Weight Marker (3µL). Lane 2: recombinant LAC-I (0.05µg). Primary Antibody: LacI Antibody at 1:1000 for 1hr at RT. Secondary Antibody: Goat anti-Rabbit IgG HRP at 1:40,000 for 30mins at RT. Block: BlockOut Buffer for 30mins at RT. Predicted/Observed MW: ~38kDa.

Applications for LacI Antibody - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kDa in size corresponding - Lac I by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LacI

The Lactose inhibitor," LacI, is a DNA-binding transcription factor that represses transcription of the operon involved in transport and catabolism of lactose.

Alternate Names

DNA-binding transcriptional repressor LacI, ECK0342

Gene Symbol

lacI

UniProt

P03023 (Human)

Additional LacI Products

Product Documents for LacI Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LacI Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for LacI Antibody - BSA Free

There are currently no reviews for this product. Be the first to review LacI Antibody - BSA Free and earn rewards!

Have you used LacI Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies