LAD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86126

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPRTISFRMKPKKENSETTLTRSASMKLPDNTVKLGEKLERYHTAIRRSESVKSRGLPCTELFVAPVGVASKRHLFEKELAGQSRAEPASSRKENLRLSGVVTSRLNLWISRTQESGDQDPQEAQKASSATERTQWGQKSDSSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LAD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LAD1 Antibody [NBP1-86126]

Immunocytochemistry/ Immunofluorescence: LAD1 Antibody [NBP1-86126]

Immunocytochemistry/Immunofluorescence: LAD1 Antibody [NBP1-86126] - Staining of human cell line MCF7 shows localization to actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126] - Staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126] - Staining of human liver shows no positivity in hepatocytes as expected.
LAD1 Antibody - BSA Free Immunohistochemistry-Paraffin: LAD1 Antibody - BSA Free [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody - BSA Free [NBP1-86126]

Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.
LAD1 Antibody - BSA Free Western Blot: LAD1 Antibody - BSA Free [NBP1-86126]

Western Blot: LAD1 Antibody - BSA Free [NBP1-86126]

Analysis in human cell lines A-431 and HEK293 using Anti-LAD1 antibody. Corresponding LAD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
LAD1 Antibody - BSA Free

Immunohistochemistry: LAD1 Antibody - BSA Free [NBP1-86126] -

Overexpression of ectopic LAD1 promotes migration and invasion of SW480 cells in vitro. a LAD1 expression increased in SW480 cells transfected with ectopic LAD1 (LAD1) compared with counterpart cells with mock vector (CTL). b and c LAD1 overexpression increased the migration and invasion of SW480 cells determined by Transwell assays. Representative images of migrated and invaded cells are shown in (b). Relative cell numbers of migrated and invaded cells per well were normalized by the numbers of CTL cells with those of LAD1 cells (c). Error bars represent the mean standard deviation (n = 3; *, p < 0.05) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33267790), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LAD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAD1

LAD1 is encoded by this gene may be an anchoring filament that is a component of basement membranes. It may contribute to the stability of the association of the epithelial layers with the underlying mesenchyme. [provided by RefSeq]

Alternate Names

LAD, Lad-1, LADA, ladinin 1, ladinin-1, linear IgA bullous dermatosis antigen, Linear IgA disease antigen, linear IgA disease antigen homolog, MGC10355

Gene Symbol

LAD1

Additional LAD1 Products

Product Documents for LAD1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LAD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LAD1 Antibody - BSA Free

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Protocols

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