Lamin A + C Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56649

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bat (94%), Bovine (94%), Chinese Hamster (100%), Ferret (100%), Mink (100%), Mouse (100%), Porcine (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lamin A + C Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 249-265 CRAQHEDQVEQYKKELEK of Human Lamin A/C was used immunogen.

Localization

Nucleus, Nucleus Envelope/Lamina

Marker

Nuclear Envelope Marker

Specificity

Because of immunogen's 100% homology with Isoforms 1, 4, 5, 6, ADelta10 and C of Prelamin-A/C, this antibody is expected to detect multiple bands in WB.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lamin A + C Antibody - BSA Free

Western Blot: Lamin A + C Antibody [NB100-56649]

Western Blot: Lamin A + C Antibody [NB100-56649]

Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NB100-56649]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NB100-56649]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NB100-56649] - Lamin A/C antibody was tested in A431 cells at 1:100 against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue).
Flow (Intracellular): Lamin A + C Antibody [NB100-56649]

Flow (Intracellular): Lamin A + C Antibody [NB100-56649]

Flow (Intracellular): Lamin A + C Antibody [NB100-56649] - Intracellular staining of HeLa cells (1 x 10^6 cells/mL) with Lamin A+C antibody (orange) stained at 1:1000. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary only control (blue).

Applications for Lamin A + C Antibody - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500 - 1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera with PBS and 50% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin A + C

Lamin A and lamin C are alternative splicing products of the lamin A/C gene that is responsible for autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD) (1). Aberrant expression patterns of nuclear lamins have been described in various types of cancer depending on the subtype of cancer, its aggressiveness, proliferative capacity and degree of differentiation. In general, the expression of A-type lamins (lamins A and C) has been correlated with a non-proliferating, differentiated state of cells and tissues. Lamins A and C, the products of the LMNA gene, are nuclear intermediate filament proteins and are the major structural components of the lamina network that underlies and supports the nuclear envelope (2).

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Entrez Gene IDs

4000 (Human); 16905 (Mouse); 44161 (Rat)

Gene Symbol

LMNA

UniProt

P02545 (Mouse)

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Lamin A + C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lamin A + C Antibody - BSA Free

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Protocols

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