Lamin A + C Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-25152

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine, Monkey

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Format

BSA Free
View all Lamin A + C Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human lamin A protein expressed in and purified from E. coli.

Localization

Nucleus. Nucleus envelope.

Marker

Nuclear Envelope Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

74/65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lamin A + C Antibody - BSA Free

Western Blot: Lamin A + C Antibody [NBP2-25152]

Western Blot: Lamin A + C Antibody [NBP2-25152]

Western Blot: Lamin A + C Antibody [NBP2-25152] - Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, NBP2-25152, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NBP2-25152]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NBP2-25152]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25152] - Analysis of HeLa cells stained with chicken pAb to lamin A/C, NBP2-25152, dilution 1:2,000 in red, and costained with mouse mAb to actin, dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. The CPCA-LaminAC antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

Applications for Lamin A + C Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:2000
Application Notes
This Lamin A + C antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where two bands can be seen at 74 kDa (Lamin A) and 65 kDa (Lamin C).

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin A + C

The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm. The Lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene, while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74kDa while Lamin C is 65kDa. The Lamin A protein includes a C-terminal segment of 98 amino acids missing from Lamin C, while Lamin C has a unique C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some Laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of Lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging.

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Entrez Gene IDs

4000 (Human)

Gene Symbol

LMNA

OMIM

150330 (Human)

UniProt

P02545 (Mouse)

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Lamin A + C Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lamin A + C Antibody - BSA Free

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Protocols

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FAQs for Lamin A + C Antibody - BSA Free

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  • Q: Could you let me know the buffer formulation of the cat# NBP2-25152?

    A: Our lab has just confirmed that NBP2-25152 is supplied in 1x PBS with 0.02% sodium azide.

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