Laminin alpha 2 Antibody (PSH0-94)

Western Blot: Laminin alpha 2 Antibody (PSH0-94) [NBP3-32521]

Western Blot: Laminin alpha 2 Antibody (PSH0-94) [NBP3-32521] - Western blot analysis of Laminin alpha 2 on different lysates with Rabbit anti-Laminin alpha 2 antibody (NBP3-32521) at 1/1,000 dilution.

Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate
Lane 3: Mouse skeletal muscle tissue lysate
Lane 4: Rat skeletal muscle tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 344 kDa
Observed band size: 344 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32521) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Laminin alpha 2 Antibody (PSH0-94) [NBP3-32521]

Immunohistochemistry: Laminin alpha 2 Antibody (PSH0-94) [NBP3-32521] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Laminin alpha 2 antibody (NBP3-32521) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32521) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: Laminin alpha 2 Antibody (PSH0-94) [NBP3-32521]

Immunohistochemistry: Laminin alpha 2 Antibody (PSH0-94) [NBP3-32521] - Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Laminin alpha 2 with Rabbit anti-Laminin alpha 2 antibody (NBP3-32521) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32521, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).