Laminin alpha 3/Laminin-5 Antibody (SR3297) - BSA Free

Novus Biologicals | Catalog # NBP3-27766

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR3297 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from human Laminin alpha 3/Laminin-5 (Uniprot #: Q16787)

Specificity

Detects endogenous levels of total Laminin alpha 3/Laminin-5

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

367 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Laminin alpha 3/Laminin-5 Antibody (SR3297) - BSA Free

Western Blot: Laminin alpha 3/Laminin-5 Antibody (SR3297) [NBP3-27766] -

Western Blot: Laminin alpha 3/Laminin-5 Antibody (SR3297) [NBP3-27766] - Western blot analysis of HeLa cell lysate.

Applications for Laminin alpha 3/Laminin-5 Antibody (SR3297) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 3/Laminin-5

The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]

Alternate Names

Epiligrin, LAMA3, Laminin-5, Laminin5

Gene Symbol

LAMA3

Additional Laminin alpha 3/Laminin-5 Products

Product Documents for Laminin alpha 3/Laminin-5 Antibody (SR3297) - BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin alpha 3/Laminin-5 Antibody (SR3297) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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