Laminin alpha 4 Antibody (CL3183) - BSA Free

Novus Biologicals | Catalog # NBP2-42392

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL3183

Format

BSA Free
View all Laminin alpha 4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q16363, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Novus Biologicals Mouse Laminin alpha 4 Antibody (CL3183) - BSA Free (NBP2-42392) is a monoclonal antibody validated for use in IHC and WB. Anti-Laminin alpha 4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Laminin alpha 4 Antibody (CL3183) - BSA Free

Western Blot: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Western Blot: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Western Blot: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Analysis of purified human recombinant Laminin-411, Laminin-421, Laminin-511, Laminin-121, Laminin-221 and Laminin-332.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of rat rectum shows immunoreactivity in the basement membrane of glandular epithelium.
Western Blot: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Western Blot: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Western Blot: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Analysis in human placenta tissue.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of human colon shows strong immunoreactivity in basement membrane of glandular epithelium.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of human pancreas shows strong immunoreactivity in basement membrane of glandular epithelium.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of human lymph node shows absence of immunoreactivity in lymphoid tissue (negative control).
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of mouse stomach shows positivity in the basement membrane of glandular epithelium.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of rat heart muscle shows membranous positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of rat epididymis shows strong immunoreactivity in lamina propria.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of mouse epididymis shows immunoreactivity in smooth muscle in the lamina propria.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of rat stomach shows immunoreactivity in the basement membrane of glandular epithelium.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of human placenta shows strong positivity in basement membrane and endothelium.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody (CL3183) [NBP2-42392] - Staining of rat kidney shows basement membrane immunoreactivity in renal tubules and glomeruli.

Applications for Laminin alpha 4 Antibody (CL3183) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 4

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cellassociated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans.Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of alpha, beta, and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human laminin alpha-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung, ovary, small and large intestines, liver, and placenta.

Alternate Names

LAMA4

Gene Symbol

LAMA4

UniProt

Q16363

Additional Laminin alpha 4 Products

Product Documents for Laminin alpha 4 Antibody (CL3183) - BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin alpha 4 Antibody (CL3183) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Citations for Laminin alpha 4 Antibody (CL3183) - BSA Free

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Protocols

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FAQs

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