Laminin alpha 4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87716

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Laminin alpha 4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ENLLNQARELQAKAESSSDEAVADTSRRVGGALARKSALKTRLSDAVKQLQAAERGDAQQRLGQSRLITEEANRTTMEVQQATAPMANNLTNWSQNLQHFDSSAYNTAVNSARDAVRNLTEVVPQLLDQLRTVEQKRPAS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Laminin alpha 4 Antibody - BSA Free (NBP1-87716) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Laminin alpha 4 Antibody - BSA Free

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716] - Staining of human smooth muscle shows high expression.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716] - Staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP1-87716] - Staining in human smooth muscle and skeletal muscle tissues using anti-LAMA4 antibody. Corresponding LAMA4 RNA-seq data are presented for the same tissues.

Applications for Laminin alpha 4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 4

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cellassociated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans.Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of alpha, beta, and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human laminin alpha-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung, ovary, small and large intestines, liver, and placenta.

Alternate Names

LAMA4

Gene Symbol

LAMA4

Additional Laminin alpha 4 Products

Product Documents for Laminin alpha 4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin alpha 4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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Protocols

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