Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71114

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B4

Format

Azide and BSA Free
View all Laminin S Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 314-561 of human LAMB2(NP_002283) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

192.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free

Western Blot: Laminin S/Laminin beta 2 Antibody (OTI3B4)Azide and BSA Free [NBP2-71114]

Western Blot: Laminin S/Laminin beta 2 Antibody (OTI3B4)Azide and BSA Free [NBP2-71114]

Western Blot: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Laminin S.
Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114]

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114]

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114]

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114]

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free [NBP2-71114] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:200

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin S

The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and gamma. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The beta-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.

Long Name

Laminin, beta 2

Alternate Names

LAMB2, Laminin beta 2

Gene Symbol

LAMB2

Additional Laminin S Products

Product Documents for Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin S/Laminin beta 2 Antibody (OTI3B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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Protocols

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FAQs

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