LCAT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-05950

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LCAT Primary Antibodies »

Product Specifications

Immunogen

Carboxyl-terminal histidine-tagged mouse recombinant LCAT purified from the medium of CHO cells overexpressing mouse LCAT cDNA. [UniProt# P16301]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LCAT Antibody - BSA Free

Western Blot: LCAT Antibody [NBP1-05950]

Western Blot: LCAT Antibody [NBP1-05950]

Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.
Immunocytochemistry/ Immunofluorescence: LCAT Antibody [NBP1-05950]

Immunocytochemistry/ Immunofluorescence: LCAT Antibody [NBP1-05950]

Immunocytochemistry/Immunofluorescence: LCAT Antibody [NBP1-05950] - The LCAT antibody was tested at a 1:500 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

Applications for LCAT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:1000
Application Notes
In Western blot, a band is seen at ~62-65 kDa representing LCAT. Also has been shown to immunodeplete LCAT from mouse plasma, suggesting it should work fine for Immunoprecipitation (PMID: 18583707).

Reviewed Applications

Read 1 review rated 5 using NBP1-05950 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LCAT

Lecithin: cholesterol acyltransferase (LCAT) is the plasma enzyme responsible for most HDL cholesteryl esters in human plasma. In species with cholesteryl ester transfer protein (CETP) it is also responsible for a significant proportion of VLDL and LDL cholesteryl esters. LCAT, through the esterification of cholesterol, plays a role in the reverse cholesterol transport pathway by facilitating the net movement of cholesterol from peripheral tissues back to the liver for excretion. LCAT deficiency results in low levels of plasma HDL, whereas a transgenic overexpression of LCAT results in increased plasma HDL concentrations.

Alternate Names

EC 2.3.1.43, lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase, phosphatidylcholine-sterol acyltransferase

Entrez Gene IDs

3931 (Human); 16816 (Mouse)

Gene Symbol

LCAT

UniProt

P04180 (Human)

Additional LCAT Products

Product Documents for LCAT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LCAT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LCAT Antibody - BSA Free

Customer Reviews for LCAT Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse liver
    Species: Mouse
    Verified Customer | Posted 11/12/2015
    mouse liver 10ug
    LCAT Antibody - BSA Free NBP1-05950

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