LCMT1 Antibody (OTI2C9)

Novus Biologicals | Catalog # NBP2-00923

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C9
View all LCMT1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LCMT1(NP_057393) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LCMT1 Antibody (OTI2C9)

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LCMT1 monoclonal antibody.
Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Staining of paraffin-embedded Human tonsil using anti-LCMT1 mouse monoclonal antibody.
Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LCMT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LCMT1.
Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LCMT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LCMT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LCMT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Staining of paraffin-embedded Human breast tissue using anti-LCMT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Staining of paraffin-embedded Human endometrium tissue using anti-LCMT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Staining of paraffin-embedded Human lymphoma tissue using anti-LCMT1 mouse monoclonal antibody.
LCMT1 Antibody (OTI2C9)

LCMT1 Antibody (OTI2C9)[NBP2-00923]

LCMT1 Antibody (OTI2C9)[NBP2-00923]Simple Western� analysis of endogenous protein TUBB4A from HELA lysates (0.2 mg/mL) using TUBB4A Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for LCMT1 Antibody (OTI2C9)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.51 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LCMT1

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM].

Alternate Names

CGI-68, EC 2.1.1.-, LCMT, leucine carboxyl methyltransferase 1, PPMT1, protein phosphatase methyltransferase 1, Protein-leucine O-methyltransferase

Entrez Gene IDs

51451 (Human)

Gene Symbol

LCMT1

Additional LCMT1 Products

Product Documents for LCMT1 Antibody (OTI2C9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LCMT1 Antibody (OTI2C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for LCMT1 Antibody (OTI2C9)

There are currently no reviews for this product. Be the first to review LCMT1 Antibody (OTI2C9) and earn rewards!

Have you used LCMT1 Antibody (OTI2C9)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies