LEF1 Antibody (LEF1/6764)
Novus Biologicals | Catalog # NBP3-20278
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LEF1/6764
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Product Specifications
Immunogen
Recombinant fragment of human LEF1 protein (exact sequence is proprietary)
Localization
Nucleus.
Specificity
Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Positive Controls: Human tonsil and thymus.
Antibody with azide store at 2 to 8C. Antibody without azide store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide store at 2 to 8C. Antibody without azide store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for LEF1 Antibody (LEF1/6764)
LEF1 Antibody (LEF1/6764)
Analysis of Protein Array containing more than 19,000 full-length human proteins using LEF1-Monospecific Mouse Monoclonal Antibody (LEF1/6764).
LEF1 Antibody (LEF1/6764)
Formalin-fixed, paraffin-embedded human tonsil stained with LEF1 antibody (LEF1/6764). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
LEF1 Antibody (LEF1/6764)
Formalin-fixed, paraffin-embedded human tonsil stained with LEF1 antibody (LEF1/6764). Inset: PBS instead of primary antibody; secondary only negative control.![LEF1 Antibody (LEF1/6764) Western Blot: LEF1 Antibody (LEF1/6764) [NBP3-20278] -](https://beta-resources.rndsystems.com/images/products/nbp3-20278_mouse-lef1-mab-lef1-6764-295202516372938.jpeg "Western Blot: LEF1 Antibody (LEF1/6764) [NBP3-20278] -")
Western Blot: LEF1 Antibody (LEF1/6764) [NBP3-20278] -
Western blot analysis of K-562 and T98G cell lysates using LEF1 Antibody (LEF1/6764).Applications for LEF1 Antibody (LEF1/6764)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: LEF1
Long Name
Lymphoid Enhancer-binding Factor 1
Alternate Names
TCF10, TCF1ALPHA, TCF7L3
Gene Symbol
LEF1
UniProt
Additional LEF1 Products
Product Documents for LEF1 Antibody (LEF1/6764)
Product Specific Notices for LEF1 Antibody (LEF1/6764)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
