LEO1 Antibody - BSA Free

Novus Biologicals | Catalog # NB600-276

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LEO1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 550 and 600 of human senescence downregulated leo1-like using the numbering given in TrEMBL entry Q8WVC0 (GeneID 123169).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LEO1 Antibody - BSA Free

Western Blot: LEO1 Antibody [NB600-276]

Western Blot: LEO1 Antibody [NB600-276]

Western Blot: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody [NB600-276] used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: LEO1 Antibody [NB600-276]

Immunohistochemistry: LEO1 Antibody [NB600-276]

Immunohistochemistry: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Leo1 (NB600-276). Detection: DAB
Western Blot: LEO1 Antibody [NB600-276]

Western Blot: LEO1 Antibody [NB600-276]

Western Blot: LEO1 Antibody [NB600-276] - Detection of human Leo1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, MCF-7, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody [NB600-276] used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: LEO1 Antibody [NB600-276]

Immunohistochemistry: LEO1 Antibody [NB600-276]

Immunohistochemistry: LEO1 Antibody [NB600-276] - Sample: FFPE section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-LEO1 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: LEO1 Antibody [NB600-276]

Immunoprecipitation: LEO1 Antibody [NB600-276]

Immunoprecipitation: LEO1 Antibody [NB600-276] - Detection of human Leo1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Leo1 antibody [NB600-276] (lot 3) used for IP at 6 ug per reaction. Leo1 was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated Leo1, NB600-276 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for LEO1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-8 ug/mg lysate

Western Blot

1:1000-1:5000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LEO1

Leo1 is one of four subunits of the Paf complex, which has been shown to aid in the regulation of transcriptional elongation, histone methylation and RNA processing in yeast.

Alternate Names

Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae), RDLReplicative senescence down-regulated leo1-like protein, RNA polymerase-associated protein LEO1

Entrez Gene IDs

123169 (Human)

Gene Symbol

LEO1

UniProt

Q8WVC0 (Human)

Additional LEO1 Products

Product Documents for LEO1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LEO1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LEO1 Antibody - BSA Free

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Protocols

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FAQs

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