Lgi1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33087

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (98%), Chimpanzee (100%), Mouse (95%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LGI1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lgi1. The exact sequence is proprietary.

Reactivity Notes

Chicken (86%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lgi1 Antibody - BSA Free

Western Blot: Lgi1 Antibody [NBP1-33087]

Western Blot: Lgi1 Antibody [NBP1-33087]

Western Blot: Lgi1 Antibody [NBP1-33087] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Lgi1 Antibody [NBP1-33087]

Western Blot: Lgi1 Antibody [NBP1-33087]

Western Blot: Lgi1 Antibody [NBP1-33087] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:5000.
Western Blot: Lgi1 Antibody [NBP1-33087]

Western Blot: Lgi1 Antibody [NBP1-33087]

Western Blot: Lgi1 Antibody [NBP1-33087] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lgi1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody [NBP1-33087] -

LGI1 antibody [N2C2], Internal detects LGI1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LGI1 protein stained by LGI1 antibody [N2C2], Internal (NBP1-33087) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Lgi1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LGI1

This gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy.

Long Name

Leucine-Rich Glioma-Inactivated Protein 1

Alternate Names

ADLTE, ADPAEF, ADPEAF, BB130740, Epitempin, Epitempin-1, EPT, ETL1, IB1099

Gene Symbol

LGI1

Additional LGI1 Products

Product Documents for Lgi1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Lgi1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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