LMO2 Antibody (4D8) - BSA Free

Novus Biologicals | Catalog # NBP2-37442

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D8

Format

BSA Free
View all LMO2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human LMO2 (AA: 1-158) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

18.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse LMO2 Antibody (4D8) - BSA Free (NBP2-37442) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LMO2 Antibody (4D8) - BSA Free

Western Blot: LMO2 Antibody (4D8)BSA Free [NBP2-37442]

Western Blot: LMO2 Antibody (4D8)BSA Free [NBP2-37442]

Western Blot: LMO2 Antibody (4D8) [NBP2-37442] - Western blot analysis using LMO2 mAb against HEK293 (1) and LMO2 (AA: 1-158)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: LMO2 Antibody (4D8) - BSA Free [NBP2-37442]

Flow Cytometry: LMO2 Antibody (4D8) - BSA Free [NBP2-37442]

Flow Cytometry: LMO2 Antibody (4D8) [NBP2-37442] - An intracellular stain was performed on U-937 cells with LMO2 Antibody (4D8) NBP2-37442F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
Western Blot: LMO2 Antibody (4D8)BSA Free [NBP2-37442]

Western Blot: LMO2 Antibody (4D8)BSA Free [NBP2-37442]

Western Blot: LMO2 Antibody (4D8) [NBP2-37442] - Western blot analysis using LMO2 mAb against human LMO2 recombinant protein. (Expected MW is 43.9 kDa)
ELISA: LMO2 Antibody (4D8) - BSA Free [NBP2-37442]

ELISA: LMO2 Antibody (4D8) - BSA Free [NBP2-37442]

ELISA: LMO2 Antibody (4D8) [NBP2-37442] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for LMO2 Antibody (4D8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMO2

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Gene Symbol

LMO2

Additional LMO2 Products

Product Documents for LMO2 Antibody (4D8) - BSA Free

Certificate of Analysis

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Product Specific Notices for LMO2 Antibody (4D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Transcription Factors

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Protocols

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FAQs

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