LOXL4 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04898

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all LOXL4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human LOXL4 (NP_115587.6). QSLGTTKLRLVGPESKPEEGRLEVLHQGQWGTVCDDNFAIQEATVACRQLGFEAALTWAHSAKYGQGEGPIWLDNVRCVGTESSLDQCGSNGWGVSDCSHSEDVGVICHPRRHRGYLSETVSNALGPQGRRLEEVRLKPILASAKQHSPVTEGAVEVKYEGHWRQVCDQGWTMNNSRVVCGMLGFPSEVPVDSHYYRKVWDLKMRDPKSRLKSLTNKNSFWIHQVTCLGTEPHMANCQVQVAPARGKLRPACPGGM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LOXL4 Antibody - Azide and BSA Free

Western Blot: LOXL4 AntibodyAzide and BSA Free [NBP3-04898]

Western Blot: LOXL4 AntibodyAzide and BSA Free [NBP3-04898]

Western Blot: LOXL4 Antibody [NBP3-04898] - Analysis of extracts of BxPC-3 cells, using LOxL4 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
LOXL4 Antibody

Immunohistochemistry-paraffin: LOXL4 Antibody [NBP3-04898] -

Immunohistochemistry-paraffin: LOXL4 Antibody [NBP3-04898] - LOXL4 was tested in Human HCC tissue for IHC. Primary Ab: LOXL4 antibody dilution at 1:100 and 1:200 (diluted in 10% goat anti rabbit serum). Secondary Ab: biotin anti rabbit (BD550880) diluted in 1:200, 0.025% PBST. Image from verified customer review.

Applications for LOXL4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

Validated from a verified customer review

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP3-04898 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LOXL4

LOXL4 encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.

Long Name

Lysyl Oxidase like 4

Alternate Names

FLJ21889, LOX L4, LOXC

Gene Symbol

LOXL4

Additional LOXL4 Products

Product Documents for LOXL4 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LOXL4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Oxidases and Oxygenases
Redox Enzymes

Customer Reviews for LOXL4 Antibody - Azide and BSA Free (1)

5 out of 5
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Customer Images

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  • LOXL4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Paraffined tumor tissue from HCC xenograft
    Species: Human
    Verified Customer | Posted 06/14/2023
    Primary Ab: LOXL4 antibody dilution tried 1:100 and 1:200 diluted in 10% goat anti rabbit serum Secondary Ab: biotin anti rabbit (BD550880): diluted in 1:200, 0.025% PBST
    Tissue Used: Formalin-fixed paraffin-embedded HCC tissue were processed for IHC DAB (BD550880): Chromogen for immunohistochemical staining
    LOXL4 Antibody - Azide and BSA Free NBP3-04898
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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