LPCAT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88921

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LPCAT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYITEEEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKIFTTYLDLQTCHVFSLPKEVQTTPSTASNKVSPE

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32140075).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LPCAT2 Antibody - BSA Free

Western Blot: LPCAT2 Antibody [NBP1-88921]

Western Blot: LPCAT2 Antibody [NBP1-88921]

Western Blot: LPCAT2 Antibody [NBP1-88921] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: LPCAT2 Antibody [NBP1-88921]

Immunocytochemistry/ Immunofluorescence: LPCAT2 Antibody [NBP1-88921]

Immunocytochemistry/Immunofluorescence: LPCAT2 Antibody [NBP1-88921] - Staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-88921 antibody. Corresponding LPCAT2 RNA-seq data are presented for the same tissues.
Western Blot: LPCAT2 Antibody [NBP1-88921]

Western Blot: LPCAT2 Antibody [NBP1-88921]

Western Blot: LPCAT2 Antibody [NBP1-88921] - Analysis in human thyroid gland tissue.
Immunocytochemistry/ Immunofluorescence: LPCAT2 Antibody [NBP1-88921]

Immunocytochemistry/ Immunofluorescence: LPCAT2 Antibody [NBP1-88921]

Immunocytochemistry/Immunofluorescence: LPCAT2 Antibody [NBP1-88921] - Staining of murine cell line RAW264.7. Image provided by product review by verified customer.
Immunohistochemistry-Frozen: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Frozen: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Frozen: LPCAT2 Antibody [NBP1-88921] - Staining of LPCAT2 in rat neuronal tissue using anti-LPCAT2 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Staining of human skeletal muscle shows very weak positivity in myocytes.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Staining of human small intestine shows weak membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Staining of human testis shows weak granular cytoplasmic positivity in Leydig cells.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921]

Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Staining of human thyroid gland shows strong granular cytoplasmic positivity in glandular cells.

Applications for LPCAT2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Frozen

Validated from verified customer review

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-88921 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LPCAT2

LPCAT2 encodes a member of the lysophospholipid acyltransferase family. The encoded enzyme may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. The encoded protein may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. The enzyme may localize to the endoplasmic reticulum and the Golgi. [provided by RefSeq]

Long Name

Lysophosphatidylcholine Acyltransferase 2

Alternate Names

AGPAT11, AYTL1, LPCAT-2

Gene Symbol

LPCAT2

Additional LPCAT2 Products

Product Documents for LPCAT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LPCAT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LPCAT2 Antibody - BSA Free

Customer Reviews for LPCAT2 Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Rat neuronal tissue
    Species: Rat
    Verified Customer | Posted 02/20/2015
    LPCAT2 staining in rat tissue
    LPCAT2 Antibody - BSA Free NBP1-88921
  • Name: Martin Helley
    Application: Immunocytochemistry
    Sample Tested: RAW264.7 cells
    Species: Mouse
    Verified Customer | Posted 02/12/2013
    RAW264.7 cells on mouse tissue
    LPCAT2 Antibody - BSA Free NBP1-88921

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Protocols

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FAQs for LPCAT2 Antibody - BSA Free

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  • Q: Do you have an estimated band size for the LPCAT2 antibody (NBP1-88921) for Western blotting?

    A: According to Uniprot, human LPCAT2 has a predicted molecular weight of approximately 60kDa. Please see UniProt entry Q7L5N7.

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