LRH-1/NR5A2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-27196

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Chinese Hamster, Equine, Guinea Pig, Opossum, Primate, Rabbit, Rhesus Macaque, Zebrafish

Cited:

Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Cited:

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LRH-1/NR5A2 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 140-190 from human NR5A2 was used as the immunogen for this antibody.

Reactivity Notes

Orangutan (100%), Chimpanzee (100%), New World Monkey (100%), Panda (100%), Great Ape (100%), Mole Rat (100%), White cheeked gibbon (100%), Platypus (100%), Zebra finch (100%), Catfish (100%), Frog (100%), Carolina anole (100%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRH-1/NR5A2 Antibody - BSA Free

Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196]

Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196]

Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2 in HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH3T3 and 4) RAW lysate probed with 3 ug/ml of NR5A2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196]

Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196]

Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2/LRH1 in human pancreas tissue using NR5A2/LRH1 antibody at 5 ug/ml.
LRH-1/NR5A2 Antibody - BSA Free

Western Blot: LRH-1/NR5A2 Antibody - BSA Free [NBP2-27196] -

Effects of NR5A2 knockdown on progesterone biosynthesis. (A) gene expression of NR5A2 after siRNA transfection; (B) effects of NR5A2 knockdown on progesterone secretion; (C) gene expression of key factors (StAR, Cyp11a1, HSD3B, and NPC1) involved in progesterone biosynthesis; (D,E) protein expression of NR5A2, StAR, Cyp11a1, and HSD3B. Primary pGCs were transfected with NR5A2 siRNA (siNR5A2) or scramble siRNA (siNC). Six hours later, the medium was changed to fresh medium with DMSO or DAPT, followed by further culture for 48 h. * means significant difference at p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31978970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LRH-1/NR5A2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5ug/ml

Knockdown Validated

reported in scientific literature (PMID 31978970)

Western Blot

1-5ug/ml~

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRH-1/NR5A2

NR5A2 is a member of the so-called NR5A family of nuclear receptors. NR5A2, also call LRH1 or Liver Receptor Homolog 1, appears to play an important role in regulating pancreatic and embryonal stem cell differentiation maintaining a pluripotential or undifferentiated state. It also plays a role in controlling cholesterol balance (1, 2). NR5A2 may control pluripotentiality through its regulation of Oct4 and Nanog, both are commonly held as requisite factors for maintenance of stem cell undifferentiated states (3). Additionally, NR5A2 has a role in colon, pancreatic and breast cancer, accompanied by overexpression in the latter in ductal carcinomas (4, 5). Antibody to NR5A2 provides a tool to link expression and function with undifferentiated states in embryonic stem cells or in cancers of the breast, intestine, and pancreas, and for further characterization of NR5A2 as a potential biomarker.

Long Name

Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2

Alternate Names

B1F2, FTZ-F1 beta, LRH1, NR5A2

Gene Symbol

NR5A2

Additional LRH-1/NR5A2 Products

Product Documents for LRH-1/NR5A2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LRH-1/NR5A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LRH-1/NR5A2 Antibody - BSA Free

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Protocols

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