LSD1 Antibody (7R10X6)

Name: LSD1 Antibody (7R10X6)
Brand: Novus Biologicals
SKU: NBP3-16688
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16688

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7R10X6 expressed in HEK293

View all Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 700-811 of human LSD1 (NP_055828.2). APILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LSD1 Antibody (7R10X6)

Western Blot: LSD1 Antibody (7R10X6) [NBP3-16688]

Western Blot: LSD1 Antibody (7R10X6) [NBP3-16688] - Western blot analysis of extracts of various cell lines, using LSD1 antibody (NBP3-16688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: LSD1 Antibody (7R10X6) [NBP3-16688]

Immunohistochemistry-Paraffin: LSD1 Antibody (7R10X6) [NBP3-16688] - Immunohistochemistry of paraffin-embedded human breast cancer using LSD1 Rabbit mAb (NBP3-16688) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for LSD1 Antibody (7R10X6)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1

LSD1 a recently identified, first known lysine-specific histone demethylase, is an 886 amino acid nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports suggest that it plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2 it serves as a novel biomarker predictive for prostate cancer.

Alternate Names

AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A

Gene Symbol

KDM1A

Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products

Product Documents for LSD1 Antibody (7R10X6)

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Certificate of Analysis

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Product Specific Notices for LSD1 Antibody (7R10X6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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