LUC7L2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-33621
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RSRHSSRDRSRERSKRRSSKERFRDQDLASCDRDRSSRDRSPRDRDRKDKKRSYESANGRSEDRRSSEEREAG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LUC7L2 Antibody - BSA Free
Immunohistochemistry-Paraffin: LUC7L2 Antibody [NBP2-33621]
Immunohistochemistry-Paraffin: LUC7L2 Antibody [NBP2-33621] - Staining of human skeletal muscle shows no positivity in myocytes as expected.Immunohistochemistry-Paraffin: LUC7L2 Antibody [NBP2-33621]
Immunohistochemistry-Paraffin: LUC7L2 Antibody [NBP2-33621] - Staining of human colon shows moderate nuclear positivity in glandular cells.Immunohistochemistry-Paraffin: LUC7L2 Antibody [NBP2-33621]
Immunohistochemistry-Paraffin: LUC7L2 Antibody [NBP2-33621] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.Immunohistochemistry-Paraffin: LUC7L2 Antibody [NBP2-33621]
Immunohistochemistry-Paraffin: LUC7L2 Antibody [NBP2-33621] - Staining of human prostate shows moderate nuclear positivity in glandular cells.Western Blot: LUC7L2 Antibody - BSA Free [NBP2-33621] -
JMJD6 forms an interaction network that consists of proteins involved in splicing and protein synthesis.(A) FLAG-tagged JMJD6 transduced into SK-N-AS cells for immunoprecipitation with anti-FLAG followed by protein identification by mass spectrometry. The interacting protein partners of JMJD6 are analyzed by STRING protein network. (B) Immunoprecipitation followed by western blot to validate the JMJD6-interacting partners in SK-N-AS cells. IP = immunoprecipitation, FT = flowthrough. n=single experiment. (C) Click-iT AHA labeling showing the newly synthesized proteins after overexpression of JMJD6 in SK-N-AS cells. n=single experiment. (D, E) Western blot showing the expression of GAC and KGA isoforms in SKNAS (D), BE2C (E), after U2AF2 and CPSF6 knockdown for 72 hr. n=single experiment.Figure 6—source data 1.Mass spectrometric analysis JMJD6 interactomes in BE2C and SKNAS cells.Mass spectrometric analysis JMJD6 interactomes in BE2C and SKNAS cells.The JMJD6 interactome in BE2C cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38488852), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for LUC7L2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LUC7L2
Alternate Names
CGI-59, CGI-74, FLJ10657, H_NH0792N18.3, hLuc7B2, LUC7B2, LUC7-like 2 (S. cerevisiae), putative RNA-binding protein Luc7-like 2
Gene Symbol
LUC7L2
Additional LUC7L2 Products
Product Documents for LUC7L2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LUC7L2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for LUC7L2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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