Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Primate

Cited:

Human, Mouse, Porcine

Predicted:

Chicken (90%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LXR alpha/NR1H3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human LXR alpha/NR1H3 protein sequence (between residues 50-150). [UniProt# Q13133]

Reactivity Notes

Immunogen sequence has 92% identity to rat and 90% identity to chicken.

Localization

Nuclear (potential)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit LXR alpha/NR1H3 Antibody - BSA Free (NB400-157) is a polyclonal antibody validated for use in WB and Simple Western. Anti-LXR alpha/NR1H3 Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LXR alpha/NR1H3 Antibody - BSA Free

Western Blot: LXR alpha/NR1H3 Antibody [NB400-157]

Western Blot: LXR alpha/NR1H3 Antibody [NB400-157]

Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.
Simple Western: LXR alpha/NR1H3 Antibody [NB400-157]

Simple Western: LXR alpha/NR1H3 Antibody [NB400-157]

Simple Western: LXR alpha/NR1H3 Antibody [NB400-157] - Simple Western lane view shows a ~49 kDa for LXR in human skeletal muscle tissue lysate under reducing conditions. Image from verified customer review.

Applications for LXR alpha/NR1H3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.5-2 ug/ml
Application Notes
This LXR alpha/NR1H3 antibody is useful for Western blot, where a band is observed at ~50 kDa.

Reviewed Applications

Read 1 review rated 3 using NB400-157 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.04 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LXR alpha/NR1H3

LXR (liver X receptors - LXR-alpha and LXR-beta, encoded by NR1H3 and NR1H2, respectively) belongs to nuclear hormone receptor superfamily and are ligand-dependent nuclear transcription factors which are activated by oxysterols. LXR beta is ubiquitously expressed whereas LXR alpha is expressed mainly in liver, intestine, adipose tissue and macrophages. LXR-alpha and LXR-beta shares 78% similarity within their ligand-binding domains and form obligate heterodimers with retinoic X receptor (RXR), bind specific response elements within promoters of target genes ABCA1, ABCG1, SREBF1, APOE etc., and regulate their expression. In the unliganded state, LXRs binds with corepressors such as N-CoR which displaces upon ligand binding. Oxysterols (oxidized cholesterol derivatives) are their natural ligands whereas endogenous ligands includes 22(R)-hydroxycholesterol (22-HC), 24(S)-hydroxycholesterol (24(S)-HC), 24,25-EC, 27-hydroxycholesterol etc. LXRs control the major metabolic pathways such as cholesterol homeostasis, lipogenesis, inflammation, toxicity proliferation cell survival/death and different aspacts of acquired/innate immunity.

Long Name

Liver X Receptor alpha

Alternate Names

NR1H3, RLD-1

Gene Symbol

NR1H3

Additional LXR alpha/NR1H3 Products

Product Documents for LXR alpha/NR1H3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LXR alpha/NR1H3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LXR alpha/NR1H3 Antibody - BSA Free

Customer Reviews for LXR alpha/NR1H3 Antibody - BSA Free (1)

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Customer Images

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  • Name: Zachary Patinkin
    Application: Simple Western
    Sample Tested: Human Skeletal Muscle Lysate
    Species: Human
    Verified Customer | Posted 06/24/2015
    Simple Western. 0.2 ug/uL human skeletal muscle tissue lysate. LXR observed at ~49 kDa.
    LXR alpha/NR1H3 Antibody - BSA Free NB400-157

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Protocols

View specific protocols for LXR alpha/NR1H3 Antibody - BSA Free (NB400-157):

LXR alpha/NR1H3 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE (4-12%) on samples to be analyzed, loading 50 ug of total protein per lane.

2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.

3. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.

4. Rinse the blot in TBS for approximately 5 minutes.

5. Block the membrane using 5% non-fat dry milk + 1% BSA in TBS for 2 hours at room temperature.

6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.

7. Dilute the rabbit anti-LXR primary antibody (NB 400-157) in blocking buffer and incubate 30 minutes at room temperature on nutator and then overnight at 4 degrees Celsius.

8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.

9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.

10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).

11. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Pierce's ECL).

Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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FAQs for LXR alpha/NR1H3 Antibody - BSA Free

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  • Q: I have a question regarding the amounts of cell extract to use with this product. You show gels from liver and skeletal muscle to demonstrate quality of the antibody, but the amounts of tissue extract used on these gels is not indicated. We are just beginning to work with this antibody, and this information would be helpful.

    A: For our product NB400-157, the blot image showing the human liver lysate was run with 25ug of human liver lysate loaded into the gel. For the other image with the human skeletal muscle lysate, this blot was performed on the ProteinSimple Wes platform. This system utilizes a capillary system to separate the proteins through instead of a traditional gel. This system therefore requires much less material. For this experiment, our lab used 5ul of lysate at 0.2mg/ml.

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