Lyn [p Tyr396] Antibody (5I5G8)

Novus Biologicals | Catalog # NBP3-16921

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5I5G8 expressed in HEK293
View all Lyn Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y396 of human Lyn (P07948). DNEYT

Modification

p Tyr396

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lyn [p Tyr396] Antibody (5I5G8)

Western Blot: Lyn [p Tyr396] Antibody (5I5G8) [NBP3-16921] - Western blot analysis of extracts of NIH/3T3 cells, using Lyn Rabbit mAb (NBP3-16921) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Lyn [p Tyr396] Antibody (5I5G8)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lyn

Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino-terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src-family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. The human Lyn gene maps to chromosome 8q13 and encodes a 505 amino acid protein. Hematopoietic tissues predominantly express Lyn, which influences normal immunoglobulin production and regulation.

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog

Alternate Names

Hck-2, JTK8, v-yes-1

Gene Symbol

LYN

Additional Lyn Products

Product Documents for Lyn [p Tyr396] Antibody (5I5G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Lyn [p Tyr396] Antibody (5I5G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

B Cell Activation
Intracellular Kinases

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