MAdCAM-1 Antibody (OTI1A5)

Novus Biologicals | Catalog # NBP2-45706

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine

Cited:

Porcine

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1A5
View all MAdCAM-1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MADCAM1(NP_570116) produced in HEK293T cell.

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:32502552).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAdCAM-1 Antibody (OTI1A5)

Western Blot: MAdCAM-1 Antibody (OTI1A5) [NBP2-45706]

Western Blot: MAdCAM-1 Antibody (OTI1A5) [NBP2-45706]

Western Blot: MAdCAM-1 Antibody (OTI1A5) [NBP2-45706] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM-1.
Immunofluorescence: MAdCAM-1 Antibody (OTI1A5) [NBP2-45706]

Immunofluorescence: MAdCAM-1 Antibody (OTI1A5) [NBP2-45706]

Immunofluorescence: MAdCAM-1 Antibody (OTI1A5) [NBP2-45706] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MADCAM1.

Applications for MAdCAM-1 Antibody (OTI1A5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:4000

Reviewed Applications

Read 1 review rated 5 using NBP2-45706 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAdCAM-1

Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or "homing receptor" for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Gene Symbol

MADCAM1

Additional MAdCAM-1 Products

Product Documents for MAdCAM-1 Antibody (OTI1A5)

Certificate of Analysis

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Product Specific Notices for MAdCAM-1 Antibody (OTI1A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MAdCAM-1 Antibody (OTI1A5)

Customer Reviews for MAdCAM-1 Antibody (OTI1A5) (1)

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  • MAdCAM-1 Antibody (OTI1A5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mammary gland tissue
    Species: Porcine
    Verified Customer | Posted 06/19/2019
    Veins and venules in the interlobular and interalveolar stroma are positive for the human MAdCAM-1.
    Antigen retrieval by a microwave method in 0.01M citric acid (pH 6.0). Alex Fluor 488 anti-mouse antibody was used as a secondary detection antibody.
    MAdCAM-1 Antibody (OTI1A5) NBP2-45706

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