MafB Antibody (OTI1E9)
Novus Biologicals | Catalog # NBP2-45718
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI1E9
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 32-320 of human MAFB (NP_005452) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
35.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MafB Antibody (OTI1E9)
![Western Blot: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Western-Blot-NBP2-45718-img0017.jpg "Western Blot: MafB Antibody (OTI1E9) [NBP2-45718]")
Western Blot: MafB Antibody (OTI1E9) [NBP2-45718]
Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0016.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human lung tissue.![Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Flow-Cytometry-NBP2-45718-img0001.jpg "Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718]")
Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718]
Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MAFB antibody.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0002.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human breast tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0003.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human pancreas tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0004.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human ovary tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0005.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Ovary tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0006.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human endometrium tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0007.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human pancreas tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0008.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human bladder tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0009.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human lymphoma tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0010.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human liver tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0011.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human breast tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0012.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human tonsil tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0013.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Kidney tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0014.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human thyroid tissue.![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]](https://resources.rndsystems.com/images/products/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0015.jpg "Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]")
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718]
Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human colon tissue.Applications for MafB Antibody (OTI1E9)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Application Notes
Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10 min is recommended
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MafB
Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B
Alternate Names
KRML
Gene Symbol
MAFB
Additional MafB Products
Product Documents for MafB Antibody (OTI1E9)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MafB Antibody (OTI1E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for MafB Antibody (OTI1E9)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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