MafB Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81342

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MafB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MEYVNDFDLLKFDVKKEPLGRAERPGRPCTRLQPAGSVSSTPLSTPCSSVPSSPSFSPTEQKTHLEDLYWMASNYQQMNPEALNLTPEDAVEALIGSHPVPQPLQSFDSFRGAHHHHHHHHPH

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25053437).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MafB Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MafB Antibody [NBP1-81342]

Immunocytochemistry/ Immunofluorescence: MafB Antibody [NBP1-81342]

Immunocytochemistry/Immunofluorescence: MafB Antibody [NBP1-81342] - Staining of human cell line hTCEpi shows localization to nucleus & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342] - Staining of human parathyroid gland shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342] - Staining of human kidney shows moderate to strong nuclear positivity in podocytes.
Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342] - Staining of human lung shows moderate to strong nuclear positivity in macrophages.
Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342]

Immunohistochemistry-Paraffin: MafB Antibody [NBP1-81342] - Staining of human pancreas shows moderate to strong nuclear positivity in islets of Langerhans.
MafB Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MafB Antibody - BSA Free [NBP1-81342]

Chromatin Immunoprecipitation-exo-Seq: MafB Antibody - BSA Free [NBP1-81342]

ChIP-Exo-Seq composite graph for Anti-MAFB (NBP1-81342) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
MafB Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MafB Antibody - BSA Free [NBP1-81342] -

Increased contractility in spinous cells of adult epidermis induces some granular markers in adult epidermis.(A and B) Immunofluorescence staining of MafB in back skin (A) or glabrous skin (B) in red, and suprabasal cells expressing Arhgef11CA marked by HA (green) in adult K10-Arhgef11 and controls. Mice were fed with doxycycline since adulthood. Insets show close-ups of HA+ cells in suprabasal layers adjacent to basal cells. Scale bars: 20 μm. (C and D) Immunofluorescence staining of Tgm1 in back skin (C) or glabrous skin (D) in red, and suprabasal cells expressing Arhgef11CA marked by HA (green) in adult K10-Arhgef11 and controls. Insets show close-ups of HA+ cells in suprabasal layers adjacent to basal cells. Mice were fed with doxycycline since adulthood. Scale bars: 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40772711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MafB Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization, Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-81342 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MafB

MAFB is encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B

Alternate Names

KRML

Gene Symbol

MAFB

Additional MafB Products

Product Documents for MafB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MafB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MafB Antibody - BSA Free

Customer Reviews for MafB Antibody - BSA Free (1)

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1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell lysate
    Species: Human
    Verified Customer | Posted 06/07/2016
    Cell lysate. Lane 1= monocytes, lane 2= monocyte-derived macrophages
    MafB Antibody - BSA Free NBP1-81342

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