MAG/Siglec-4a Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-17201

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAG/Siglec-4a Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAG/Siglec-4a. The exact sequence is proprietary.

Localization

Membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAG/Siglec-4a Antibody - BSA Free

Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP2-17201]

Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP2-17201]

Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP2-17201] - Rat fore brain. MAG antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Frozen: MAG/Siglec-4a Antibody [NBP2-17201]

Immunohistochemistry-Frozen: MAG/Siglec-4a Antibody [NBP2-17201]

Immunohistochemistry-Frozen: MAG/Siglec-4a Antibody [NBP2-17201] - Frozen-sectioned adult mouse cerebellum. Green: MAG protein stained by MAG antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500.Blue: Fluoroshield with DAPI.
MAG/Siglec-4a Antibody - BSA Free

Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] -

Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody (NBP2-17201) diluted at 1:1000.
MAG/Siglec-4a Antibody - BSA Free

Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] -

Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody (NBP2-17201) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MAG/Siglec-4a Antibody - BSA Free

Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] -

Boiled and unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody (NBP2-17201) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for MAG/Siglec-4a Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAG/Siglec-4a

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Long Name

Myelin-associated Glycoprotein

Alternate Names

Siglec-4a, Siglec4a

Gene Symbol

MAG

UniProt

P20916

Additional MAG/Siglec-4a Products

Product Documents for MAG/Siglec-4a Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAG/Siglec-4a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Glial Lineage Markers

Citations for MAG/Siglec-4a Antibody - BSA Free

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Protocols

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FAQs

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