MAGEA1 Antibody (MA454)

Novus Biologicals | Catalog # NBP2-29469

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MA454
View all MAGEA1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MAGEA1 protein (Uniprot: P43355)

Localization

Cytoplasmic

Specificity

Recognizes a protein of 42-46kDa, identified MAGEA1 (MAGE-1). This monoclonal antibody does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGEA1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33094)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MAGEA1 Antibody (MA454)

Immunohistochemistry-Paraffin: MAGEA1 Antibody (MA454) [NBP2-29469]

Immunohistochemistry-Paraffin: MAGEA1 Antibody (MA454) [NBP2-29469]

Immunohistochemistry-Paraffin: MAGE 1 Antibody (MA454) [NBP2-29469] - Formalin-fixed, paraffin-embedded human testis stained with MAGE-1 antibody (1:200), peroxidase-conjugate and DAB chromogen.

Applications for MAGEA1 Antibody (MA454)

Application
Recommended Usage

ELISA

1-5ug/ml for coating

Immunohistochemistry

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Sandwich -ELISA reported in scientific literature (PMID:31640756).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MAGEA1

MAGE-1 is a cancer testis antigen (CTA) expressing in a variety of human cancers (Pereira CM et al., 2012). It is a human melanoma antigen recognized by cytolytic T lymphocytes. MAGE-1 induces a tumor specific immune response so it is thought to be a potential therapeutic target for cancer immunotherapy (Ma ZY et al., 2002). The gene for MAGE-1 belongs to a family of 12 genes known as MAGE-A cluster located on the X chromosome in region q28 (Laduron S et al., 2004). This relies primarily on DNA methylation for repression in somatic tissues but in many types of tumors, the promoter of these genes becomes demethylated and transcription becomes activated (Loriot A et al., 2006). Monoclonal antibody aganist a recombinant feline melanoma antigen 1 (fMAGE-1) has been generated and is effective against feline lymphoma cell lines and tumor tissues (Ma ZY et al., 2002). Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.

Long Name

Melanoma-Associated Antigen 1

Alternate Names

CT1.1, MAGE-A1, MAGE1, MGC9326, MZ2-E

Gene Symbol

MAGEA1

UniProt

P43355 (Human)

Additional MAGEA1 Products

Product Documents for MAGEA1 Antibody (MA454)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MAGEA1 Antibody (MA454)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Stem Cell Markers

Citations for MAGEA1 Antibody (MA454)

Customer Reviews for MAGEA1 Antibody (MA454)

There are currently no reviews for this product. Be the first to review MAGEA1 Antibody (MA454) and earn rewards!

Have you used MAGEA1 Antibody (MA454)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies