MALT1 Antibody (MT1/410)

Novus Biologicals | Catalog # NBP2-29430

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MT1/410
View all MALT1 Primary Antibodies »

Product Specifications

Immunogen

Human MALT1 recombinant fragment (aa701-808) (Uniprot: Q9UDY8)

Localization

Cytoplasmic

Marker

MALT-Lymphoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33054)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MALT1 Antibody (MT1/410)

Western Blot: MALT1 Antibody (MT1/410) [NBP2-29430]

Western Blot: MALT1 Antibody (MT1/410) [NBP2-29430]

Western Blot: MALT1 Antibody (MT1/410) [NBP2-29430] - Western Blot Analysis of Daudi cell Lysate using MALT1 Monoclonal Antibody (MT1/410)
Immunohistochemistry-Paraffin: MALT1 Antibody (MT1/410) [NBP2-29430]

Immunohistochemistry-Paraffin: MALT1 Antibody (MT1/410) [NBP2-29430]

Immunohistochemistry-Paraffin: MALT1 Antibody (MT1/410) [NBP2-29430] - Formalin-fixed, paraffin-embedded tonsil stained with MALT1 Ab (MT1/410)
Immunohistochemistry-Paraffin: MALT1 Antibody (MT1/410) [NBP2-29430]

Immunohistochemistry-Paraffin: MALT1 Antibody (MT1/410) [NBP2-29430]

Immunohistochemistry-Paraffin: MALT1 Antibody (MT1/410) [NBP2-29430] - Formalin-fixed, paraffin-embedded human tonsil stained with MALT1 MT1/410 antibody (1:200), peroxidase-conjugate and DAB chromogen.
Simple Western: MALT1 Antibody (MT1/410) [NBP2-29430]

Simple Western: MALT1 Antibody (MT1/410) [NBP2-29430]

Simple Western: MALT1 Antibody (MT1/410) [NBP2-29430] - Simple Western lane view shows a specific band for MALT1 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: MALT1 Antibody (MT1/410) [NBP2-29430]

Simple Western: MALT1 Antibody (MT1/410) [NBP2-29430]

Simple Western: MALT1 Antibody (MT1/410) [NBP2-29430] - Electropherogram image of the corresponding Simple Western lane. MALT1 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

Applications for MALT1 Antibody (MT1/410)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 105 kDa.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MALT1

MALT1/Paracaspase (MALT1) was independently identified as a member of the human paracaspase family and an interacting partner of B-cell lymphoma (Uren et al., 2000). MALT1 is a caspase-like protein that contains an N-terminal death domain, two Ig-like domains, and a C-terminal caspase-like domain. It binds to Bcl-10 through its Ig-like domains and cooperates with Bcl-10 to activate NF-kappaB. MALT1 is thought to play an important role in NF-kappaB signaling by enchancing NF-kappaB activation through interaction with Bcl-10 (Ho et al., 2005). Interaction between MALT1 and Bcl-10 mediates IKK activation in vitro through facilitatiing the ubiquitination of NEMO by the ubiquitin-conjugating enzyme UBC13.

Long Name

Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1

Alternate Names

MLT1, Paracaspase

Gene Symbol

MALT1

UniProt

Q9UDY8 (Human)

Additional MALT1 Products

Product Documents for MALT1 Antibody (MT1/410)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MALT1 Antibody (MT1/410)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

C-type Lectin Receptors
NFkB Pathway

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MALT1 Antibody (MT1/410)

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  • Q: NBP2-29430 has been tested for flow. What type of stainings (surface or intracellular) was tested to confirm this Ab work for flow in your company?

    A: MALT1 is a cytoplasmic protein, so you will have to do intracellular staining.

  • Q: What's difference between NBP2-32872-0.1mg and NBP2-29430?

    A: NBP2-32872-0.1mg and NBP2-29430 were raised against the same immunogen but are different clones, so they recognize a different epitope. NBP2-32872 is protein A purified, and NBP2-29430 is protein G purified.

  • Q: NBP2-29430 has been tested for flow. What type of stainings (surface or intracellular) was tested to confirm this Ab work for flow in your company?

    A: MALT1 is a cytoplasmic protein, so you will have to do intracellular staining.

  • Q: What's difference between NBP2-32872-0.1mg and NBP2-29430?

    A: NBP2-32872-0.1mg and NBP2-29430 were raised against the same immunogen but are different clones, so they recognize a different epitope. NBP2-32872 is protein A purified, and NBP2-29430 is protein G purified.

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