MAP3K9 Antibody

Novus Biologicals | Catalog # NBP3-48460

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAP3K9. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP3K9 Antibody

MAP3K9 Antibody

Western Blot: MAP3K9 Antibody [NBP3-48460] -

MAP3K9 antibody detects MAP3K9 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K9 antibody (NBP3-48460) diluted by 1:1000.
MAP3K9 Antibody

Immunocytochemistry/ Immunofluorescence: MAP3K9 Antibody [NBP3-48460] -

MAP3K9 antibody detects MAP3K9 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP3K9 protein stained by MAP3K9 antibody (NBP3-48460) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for MAP3K9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP3K9

MLK1 is a MLK(MAP3K) type protein kinase. In vitro studies suggest that it is a member of the neuronal apoptotic JNK/c-Jun pathway acting between Rac1/Cdc42 and MKK4 and -7 in death signaling. MLK1 expression has been documented in human epithelial tumor cell lines of colonic, breast and esophageal origin. ESTs have been isolated from several tissue libraries, including normal pancreas and spleen and cancerous pancreas and bladder.

Alternate Names

EC 2.7.11, EC 2.7.11.25, MEKK9, mitogen-activated protein kinase kinase kinase 9, MLK1Mixed lineage kinase 1, PRKE1mixed lineage kinase 1 (tyr and ser/thr specificity)

Gene Symbol

MAP3K9

Additional MAP3K9 Products

Product Documents for MAP3K9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MAP3K9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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